In Prolog, the == operator is used for unification, which is the process of making two terms equivalent. This means that when using ==, Prolog will attempt to find substitutions for variables in order to make two terms match.On the other hand, the = operator is used for assertion, which is the process of assigning a value to a variable or structure. When using =, Prolog will assign the right-hand side term to the left-hand side variable or structure.